Barrett, K.; McGrowder, Donovan A.; Brown, Paul D.; Ragoobirsingh, Dalip
Author Affiliation, Ana.
Departement of Basic Medical Sciences
Insulin-Stimulated Glucose Uptake is Inhibited in Adipocytes [Abstract]
West Indian Medical Journal
Date of Publication
Objective: To investigate the role of nitric axide on insulin-related D-glucose-[1-3H(N)] uptake in adipo-cytes from streptozotocin-treated diabetic rats. Method: Sixteen adult Wistar rats weighing between 120-300 g were divided into two groups consisting of eight rats. The drug-treated group was injected with 65 mg/kg body weight streptozotocin (STZ) in conjunction with 180 mg/kg body weight nicotinamide, to include Type 2 diabetes mellitus. Control rats were injected with saline. After four weeks, an oral glucose tolerance test was performed and control and diabetic rats sacrificed. Blood was collected for insulin determination and D-glucose-[1-3H(N)] uptake into adipocytes inthe presence and absence of insulin measured. Conclusion: The results of the study indicate that the NO-releasing drug has direct inhibitory effect on insulin-stimulated glucose uptake in adipocytes and may thereby have a role in the aetiology of Type 2 diabetes melitus.....