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Publication Type
Journal Article
Author, Analytic
Barrett, Karlene; McGrowder, Donovan; Brown, Paul D; Ragoobirsingh, Dalip
Author Affiliation, Ana.
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Article Title
Increased PC -1 phosphodiesterase activity and inhibition of glucose uptake in adipocytes of type 2 diabetic rats
Medium Designator
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Connective Phrase
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Journal Title
Molecular and Cellular Biochemistry
Translated Title
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Reprint Status
Refereed
Date of Publication
2006
Volume ID
293
Issue ID
1-2
Page(s)
9-14
Language
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Connective Phrase
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Location/URL
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ISSN
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Notes
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Abstract
This study was designed to understand the cellular mechanisms responsible for defects in the insulin-stimulated signal transduction pathway in a type 2 diabetic animal model. We examined the in vitro PC-1 phosphodiesterase activity and glucose uptake in adipose tissue of streptozotocin (STZ)-induced type 2 diabetic rats. The PC-1 activity was significantly increased in adipose tissue of diabetic rats (0.54 0.08 nmol PNTP hydrolyzed/mg protein/min) compared with controls (0.29 0.05 nmol PNTP hydrolyzed/mg protein/min, p < 0.05). Upon insulin stimulation (100 nM), glucose uptake in the adipose tissue of the controls (4.17 1.2810-8 ?mol/mg/min) was significantly higher than that in the diabetic rats (1.26 0.3510-8; p < 0.05). These results suggest that elevated PC-1 phosphodiesterase activity and decreased glucose uptake in adipose tissues may be acquired characteristics contributing to the development of type 2 diabetes mellitus.....
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