McGrowder, Donovan; Barrett, K; Brown, Paul D; Ragoobirsingh, Dalip
Author Affiliation, Ana.
Exogenous Nitric Oxide Inhibits Glucose Uptake by peripheral tissues in a Diabetic Rat Model
Date of Publication
The study investigated the effect of nitric oxide (NO) released from S-nitrosoglutathione (GSNO) and S-nitroso-Nacetylpenicillamine (SNAP) on insulin-stimulated glucose uptake in adipocytes, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity in skeletal muscle strips of streptozotocin-induced type 2 diabetic and normoglycemic rats. GSNO at concentrations of 10 mM and 20 mM significantly decreased insulin-stimulated 2-deoxyglucose uptake by 60.9▒5.6% and 62.5▒4.6%, respectively (p<0.05), in adipocytes of diabetic rats. Similarly, SNAP at concentrations of 10 mM and 20 mM significantly decreased insulin-stimulated 2-deoxyglucose uptake by 65.6▒6.1% and 67.2▒7.2%, respectively (p<0.05). The GAPDH activity in skeletal muscle of diabetic rats (0.064▒0.006 ýmol/mg/min) was significantly lower than that in normoglycemic rats (0.110▒0.010 ýmol/mg/min; p<0.05). These results suggest that exogenous NO inhibits insulin stimulated glucose uptake in adipocytes of streptozotocin-induced type 2 diabetic rats, and this could lead to further pathologic sequels in the diabetic state.....