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Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Lamm, A.S; Chen, A.R.M; Reynolds, W.F; Reese, Paul .B
Author Affiliation, Ana.
n/a
Article Title
Steroid hydroxylation by Whetzelinia sclerotiorum, Phanerochaete chrysosporium and Mucor plumbeus
Medium Designator
n/a
Connective Phrase
n/a
Journal Title
Steroids
Translated Title
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Reprint Status
Refereed
Date of Publication
2007
Volume ID
72
Issue ID
9-10
Page(s)
713-722
Language
n/a
Connective Phrase
n/a
Location/URL
n/a
ISSN
n/a
Notes
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Abstract
The fungi Whetzelinia sclerotiorum ATCC 18687, Phanerochaete chrysosporium ATCC 24725 and Mucor plumbeus ATCC 4740 were examined for their ability to perform steroid biotransformations under single phase, pulse feed conditions. The steroids 3-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) (1), 17-hydroxyandrost-4-en-3-one (testosterone) (5), 3-hydroxypregn-5-en-20-one (pregnenolone) (3), pregn-4-ene-3,20-dione (progesterone) (9),17* ,21-dihydroxypregn-4-ene-3,11,20-trione (cortisone) (11), 17*, 21-dihydroxypregna-1,4-diene-3,11,20-trione (prednisone) (14) and 3-hydroxyestra-1,3,5(10)-trien-17-one (estrone) (15) were fed to each fungus. The production of a number of novel metabolites is reported. Of the fungi investigated W. sclerotiorum performed the most interesting biotransformations and had a clear propensity for 2, 6/7 and 15/16 hydroxylations. P. chrysosporium was more prone functionalize steroids in the allylic position. Oxygen insertion at C-14 by M. plumbeus is reported for the first time. All three micro-organisms exhibited redox activity.....
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