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Publication Type
Conference Proceedings
Author, Analytic
Wright, R.; Asemota, Helen N.; Wheatley, Andrew O.
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Paper/Section Title
Extraction of Organic Compounds from Moringa Oleifera Leaves & its Antioxidant Activity Using DPPH Assay
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Proceedings Title
9th International Symposium in Recent Advances in Environmental Health Research
Date of Meeting
September 16-18, 2012
Place of Meeting
Jackson Marriott Hotel, Jackson, Mississippi, USA
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Location/URL
http:; ehr.cset.jsums.edu/9cd/StudentPdf/Student%2041.pdf
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Abstract
Persons with sickle cell anemia (SCA) have increased oxidative stress which leads to chronic inflammation and organ damage which in turn reduces life expectancy. Antioxidants cause a reduction in oxidative stress. Moringa oleiefera leaves are a potential source of antioxidants. Extracts from Moringa oleifera leaves (grown in Jamaica) were evaluated for anti-oxidant activity of extracts. Leaf extracts were prepared using non-polar and polar solvents for a total of 7 fractions. Leaf extracts were prepared and purified based on solvent polarity using hexane, chloroform, butanol and water. Each fractionís antioxidant activity was tested using 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assay and the extracts were labeled E, E1-E5 and A. A standard curve of Trolox (a vitamin E analogue) was generated to calculate the antioxidant activity. The percentage of DPPH reduced and IC 50 (the amount of antioxidant needed to reduce 50% of DPPH) were calculated from mean absorbance values. Extracts E1 and E2 had IC 50 values of 1423?g/ml and 4458?g/ml respectively, while extracts E, E3, E4, E5 and A had approximately 2 to 6 times lower IC 50v alues of 835?g/ml, 177?g/ml, 1100?g/ml, 524?g/ml and 1004?g/ml respectively. Lower IC 50 values correlate with greater antioxidant activity of the extract; therefore extracts E3 and E5 had the best antioxidant activities. Extracts E3 and E5 were prepared from the most polar solvents used, 1-butanol and water respectively. In general, extracts prepared using polar solvents (E, E3, E4, E5 and A) had better antioxidant activity (lower IC 50) compared with those from non-polar solvents (E1 and E2). These extracts therefore may have clinical applications in diseases where there is a chronic state of oxidative stress.....
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