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Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Peart, P.C; McCook, K.P; Russell, F.A.; Reynolds, W.F.; Reese,Paul B.
Author Affiliation, Ana.
n/a
Article Title
Hydroxylation of steriods by Fusarium oxysporum,Exophiala jeanselmei and Ceratocystis paradoxa
Medium Designator
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Connective Phrase
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Journal Title
Steriods
Translated Title
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Reprint Status
Refereed
Date of Publication
2011
Volume ID
76
Issue ID
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Page(s)
1317 - 1330
Language
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Connective Phrase
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Location/URL
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ISSN
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Notes
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Abstract
The potential of Fusarium oxysporum var. cubense UAMH 9013 to perform steroid biotransformations was reinvestigated using single phase and pulse feed conditions. The following natural steroids served as substrates: dehydroepiandrosterone (1), pregnenolone (2), testosterone (3), progesterone (4), cortisone (5), prednisone (6), estrone (7) and sarsasapogenin (8). The results showed the possible presence of C-7 and C-15 hydroxylase enzymes. This hypothesis was explored using three synthetic androstanes: androstane-3,17-dione (9), androsta-4,6-diene-3,17-dione (10) and 3,5-cycloandrost-6-en-17-one (11). These fermentations of non-natural steroids showed that C-7 hydroxylation was as a result of that position being allylic. The evidence also pointed towards the presence of a C-15 hydroxylase enzyme. The eleven steroids were also fed to Exophialajeanselmei var. lecanii-corni UAMH 8783. The results showed that the fungus appears to have very active 5 and 14-hydroxylase enzymes, and is also capable of carrying out allylic oxidations. Ceratocystis paradoxa UAMH 8784 was grown in the presence of the above-mentioned steroids. The results showed that monooxygenases which effect allylic hydroxylation and Baeyer-Villiger rearrangement were active. However, redox reactions predominated....
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